Processes and Methods (incl. Screening)

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A genetically conserved and transferable defense mechanism protects plants from herbivores

Ref.-No.: 1602-6322-LI

Processes and Methods (incl. Screening) : Life Sciences-HTS/HCS
Nucleic Acid-, Protein and Cell-related Technologies : Protein related
Green Biotech

Our scientists have identified a conserved defense mechanism covering a new metabolite in tobacco that protects against leafhoppers. They have discovered how to reconstitute it in vitro and engineer the compound in crop plants, demonstrating that it confers resistance to herbivores.

A high-throughput compatible workflow for generation and analysis of homogenous human neural microtissues

Ref.-Nr.: 1012-5677-LI

Processes and Methods (incl. Screening)

File no.: MI-1012-5677-LI-ZE
(liked to MI-1012-4430-LI-ZE describing the generation of neuro- epithelial stem cells as precursors for microtissue generation)

A robust method for producing and analyzing highly homogenous human neural organoids.

A method for the production of plants with altered photorespiration and improved CO2 fixation

Ref.-No.: 0706-5666-IKF

Processes and Methods (incl. Screening) : Chemical
Processes and Methods (incl. Screening) : Life Sciences-HTS/HCS
Green Biotech

Recently, many efforts have been made to improve growth and yield of crop plants. CO2 fixation in plants is primarily catalyzed by the enzyme ribulose-1,5-bisphosphate carboxylase (Rubisco). The inherent inefficiency of Rubisco necessitates an additional metabolic process termed photorespiration, which results in the loss of carbon and energy. In fact, it has been estimated that plants lose approximately 25% of the fixed carbon due to photorespiration. Despite several attempts to improve ...

Air-stable binary Ni(0)–olefin (pre)catalyst

Ref.-Nro: 0042-5881-LC

Processes and Methods (incl. Screening) : Chemical
New Materials

A family of 16-electron binary Ni(0)-stilbene complexes Ni(Rstb)3 as a new class of air-stable Ni(0) (pre)catalysts was developed.

All-in-one nanoprinting approach for the synthesis of unclonable anti-counterfeiting nanofilms

Ref.-No.: 0401-6646-MG

Processes and Methods (incl. Screening) : Large Scale Production

In addition to causing trillion-dollar economic losses every year, counterfeiting threatens human health, social equity and national security. Current materials for anti-counterfeiting labelling typically contain toxic inorganic quantum dots and the techniques to produce unclonable patterns require tedious fabrication or complex readout methods. Carbon dots (CDs) stand out because of their stability, low toxicity, widely available precursors and bio-/ecofriendly preparation. However, most reported ...

Application of novel channelrhodopsins: molecular engineering, site-specific opsin targeting, discovery of new variants and the advancement in optogenetic devise development

Ref.-No.: 0601-4259-MG

Processes and Methods (incl. Screening) : Life Sciences-HTS/HCS
Medicine : Therapeutics
Research Tools : Other
Nucleic Acid-, Protein and Cell-related Technologies : Cell related

The development of the cation channel channelrhodopsin-2 (ChR2) from Chlamydomonas reinhardtii by Prof. Ernst Bamberg and his colleagues and its application to light-induced modulation of neurons paved the way for the field of optogenetics.
Over time the light-gated cation channel channelrhodopsin-2 (ChR2) has become an indispensable tool in neuroscience. Based on the pioneering work on ChR2 new variants have emerged, differing in their spectrum of light absorption, their kinetic ...

Automated real time determination of crystal size and 3D shape distribution to quantify and predict the properties and dynamics of the crystallization processes

Ref.-No.: 1402-5690-LC

Analytics
Processes and Methods (incl. Screening)

To monitor crystallization processes a special camera setup to measure the crystal size and 3D shape distribution was developed.
The setup consists of two high-speed cameras that are positioned orthogonally to one another such that a precise overlap of both fields of view can be achieved. The setup further contains a specially designed flow-through cell that allows to observe the crystal suspension from both camera directions and given the opportunity to temperate the solution within the cell. ...

Core-shell catalyst design for improved heat management with highly exothermic reactions

Ref.-No.: 1402-5768-LC

Processes and Methods (incl. Screening)
New Materials

Prof. Kai Sundmacher and co-workers at the MPI for Dynamics of Complex Technical Systems developed a novel core-shell catalyst design with a highly active catalyst core surrounded by an inert, low-permeability shell. The new catalyst design leads to an intrinsic heat management that avoids hotspots in strongly exothermic reactions. This allows faster reactor start-up and shut-down and makes the catalysts suitable for rapid load changes in discontinuous processes.

Exergy Efficient Chlorine Separation

Ref.-No.: 1402-5465-LC

Processes and Methods (incl. Screening) : Chemical

Three novel processes to separate Cl2 from unconverted HCl in the anode outlet stream of a electrochemical gas-phase reactor were developed at the MPI for Dynamics of Complex Technical Systems. The novel processes lead to a higher reaction yield in gas-phase reactor and have an reduced energy demand compared to the Bayer UhDenora state-of-the-art process.

Extraction of nanocellulose from cellulose pulp in reactive eutectic media

Ref.-No.: 0401-5970-LC

Processes and Methods (incl. Screening)
New Materials

A novel green chemistry process to prepare nanocellulose in efficient, safe and sustainable manner based on the use of eutectic, highly concentrated ionic media is offered.

f-Chrimson and vf-Chrimson, two ultrafast variants of the red-light activated channelrhodopsin Chrimson

Ref.-No.: 0601-5208-MG

Processes and Methods (incl. Screening) : Life Sciences-HTS/HCS
Medicine : Therapeutics
Research Tools : Other
Nucleic Acid-, Protein and Cell-related Technologies : Cell related

The development of the cation channel channelrhodopsin-2 (ChR2) from Chlamydomonas reinhardtii by Prof. Ernst Bamberg and his colleagues, and its application to light-induced modulation of neurons paved the way for the field of optogenetics.
Over time the light-gated cation channel channelrhodopsin-2 (ChR2) has become an indispensable tool in neuroscience. Based on the pioneering work on ChR2 new variants have emerged, differing in their spectrum of light absorption, their kinetic ...

Fabrication of fluid filled metal microarchitectures

Ref.-No.: 0041-6492-WT

Processes and Methods (incl. Screening)
New Materials

LEL technology stands out for its precision in fabricating 3D metal microarchitectures with intricate geometries and the ability to seamlessly integrate liquids. It utilizes a voxel-by-voxel deposition method, which allows for the creation of complex shapes with submicron resolution. The process involves optimizing variables like deposition voltage, air pressure, and orifice diameter. A significant advantage of LEL is its capacity to alter the pH and composition of the supporting electrolyte solution, ...

Fast and efficient protein purification form prokaryotic and eukaryotic hosts

Ref.-No.: 0109-4896-LI

Processes and Methods (incl. Screening)

Fusion Tags Comprising Ubiquitin-like Modifiers and Corresponding Proteases For Protein Purification From Selected Eukaryotic Expression Hosts

Free Standing Dry and Stable Nanoporous Polymer Films Made through Mechanical Deformation

Ref.-No.: 0903-6483-LC

Processes and Methods (incl. Screening)
New Materials

Nanoporous membranes consist of pores with diameters in the range of nanometers and sub nanometers that can separate liquid or gaseous mixtures.
One of the key applications of nanoporous membranes is in water filtration to remove impurities, such as bacteria, viruses, and salts. Nanoporous membranes are also used in energy-related applications, such as gas separation and fuel cells. The membranes can also be used as electrodes in energy storage devices such as batteries and supercapacitors. ...

Generation of induced pluripotent stem cells with one or two reprogramming factors

Ref.-No.: 1012-3916-LI

Processes and Methods (incl. Screening) : Life Sciences-HTS/HCS
Nucleic Acid-, Protein and Cell-related Technologies : Cell related

The cellular identity of mature cells that are confined in a specialized genetic expression profile can be overcome by introducing just a few genes that encode for so-called reprogramming factors [1]. By means of this intervention, also known as reprogramming, the cells then become pluripotent and represent induced pluripotent stem cells (iPS cells) [1]. Nowadays, numerous new cell culture models have been established based on reprogramming protocols that yield iPS cells. Such cell culture models ...

Genome-Editing-Technologies - Cas9 double mutant with improved specificity compared to wild-type

Ref.-No.: 0306-5787-LI

Processes and Methods (incl. Screening)
Medicine
Research Tools
Nucleic Acid-, Protein and Cell-related Technologies

The RNA-programmable DNA-endonuclease Cas9 is widely used for genome engineering, where a high degree of specificity is required. Though this is a mechanism well controlled by canonical base-pairing, there is evidence that Cas9 does accept mismatches under certain circumstances.
This might be seen as a threat to precision needed in genome engineering at least in environments where such high precision is ultimately needed and acceptance of mismatches needs to be avoided, e.g.: cellular gene ...

Genome-Editing-Technologies - Single compound or compound mix dramatically improve Homology-Directed-Repair (HDR)

Ref.-No.: 1306-5734-LI & 1306-5288-LI

Processes and Methods (incl. Screening)
Medicine
Research Tools
Nucleic Acid-, Protein and Cell-related Technologies

Precise genome editing demands for homology-directed repair (HDR) by aiming to introduce a new double stranded DNA substituting for a homologous sequence but partially defect one. This substrate dependent reaction does compete with direct non-homologous end joining (NHEJ) of the double strand breaks. NHEJ is error-prone and is therefor mainly used for gene inactivation.
The rate of NHEJ is directly limiting the rate of HDR. For this reason HDR in vitro does occur on a very low level only and ...

Glue your proteins of interest together: Fast and ultra-specific enzymatic conjugation of a protein with another, synthetic compounds or even whole cells

Ref.-No.: 0109-5804-LI

Processes and Methods (incl. Screening) : Life Sciences-HTS/HCS
Research Tools : Other
Nucleic Acid-, Protein and Cell-related Technologies : Protein related
Nucleic Acid-, Protein and Cell-related Technologies : Cell related

Connectase is an enzyme that fuses target proteins or synthetic compounds via a small recognition tag, using a different reaction mechanism compared to known protein ligases. It prevents side reactions entirely and has absolute specificity, even in highly in complex solutions with minimum concentrations of target molecules.

GOLD gRNA - allows robust genome editing regardless of spacer sequence composition by super stable hairpin technology

Ref.-No.: 1306-6101-LI

Processes and Methods (incl. Screening)
Medicine
Research Tools
Nucleic Acid-, Protein and Cell-related Technologies

A Cas nuclease such as Cas9 can introduce a DNA double strand break in a sequence target that is complementary to a 20-nt spacer sequence of its bound guide RNA (gRNA) when a protospacer adjacent motif (PAM) is present. The gRNA can be provided as duplex of spacer containing CRISPR RNA (crRNA) and trans-activating crRNA (tracrRNA). Or as a single gRNA (sgRNA) where crRNA and tracrRNA are fused by an artificial loop.
Hence a gRNA consists of the target-specific spacer and a constant part typically ...

High-precision base editors for site-specific single nucleotide conversion

Ref.-No.: 0402-5782-MG

Processes and Methods (incl. Screening)

The CRISPR-Cas system is a powerful tool for site-specific genome editing. It allows for the inactivation or substitution of entire genes. However, most hereditary diseases in humans are caused by single point mutations, the correction of which requires only subtle changes to the DNA. Recently, base editors (BEs) were developed that allow the introduction of selective nucleotide substitutions. A Cas9 enzyme is fused to a nucleobase deaminase catalysing C-to-T mutations (by C-to-U deamination) ...

Homogenous, TR-FRET-based method for measurement of RNA- and DNA-polymerase activity

Ref.-No.: 1013-4990-IKF

Analytics
Processes and Methods (incl. Screening) : Life Sciences-HTS/HCS
Nucleic Acid-, Protein and Cell-related Technologies : Protein related
Nucleic Acid-, Protein and Cell-related Technologies : Cell related

An innovative high throughput screening method for measurement of RNA- and DNA-polymerase activity at room temperature, based on time-resolved FRET.

LITESEC - Light-controlled protein delivery into eukaryotic cells with high spatial and temporal resolution

Ref.-No.: 0706-5707-IKF

Processes and Methods (incl. Screening) : Life Sciences-HTS/HCS
Medicine : Drug delivery
Nucleic Acid-, Protein and Cell-related Technologies : Protein related

The LITESEC-T3SS (Light-induced translocation of effectors through sequestration of endogenous components of the T3SS) system represents a novel, fast, specific, and reversible method to control protein secretion into eukaryotic cells, enabling various medicinal and biotechnological applications, such as targeted drug delivery for tumor therapy.

Method for cell-free protein synthesis assays or other fluorescent assays in the context of cell-free protein synthesis

Ref.-No.: 0204-5479-IKF

Processes and Methods (incl. Screening) : Life Sciences-HTS/HCS
Research Tools : Other
Nucleic Acid-, Protein and Cell-related Technologies : Protein related

An improved method to perform cell-free protein synthesis (CFPS) assays or other fluorescent assays in the context of CFPS with very low reaction mix volumes. Various additional advantages allow to significantly reduce costs and time.

Method of producing autotrophic microorganisms with altered photorespiration and improved CO2 fixation

Ref.-No.: 0706-5602-IKF

Processes and Methods (incl. Screening) : Chemical
Processes and Methods (incl. Screening) : Life Sciences-HTS/HCS

Biological fixation of CO2 is an important process carried out by plants and several microorganisms, which can be harnessed for sustainable, biobased production of fuels and chemicals. Particularly, the fixation of CO2 by autotrophic microorganisms such as cyanobacteria and microalgae can be employed for converting CO2 into value-added products, such as commodity chemicals or fuels. However, the sustainable autotrophic production ...

Molecularly defined non-infectious synthetic virus-like particles to mimic biophysical features of natural pathogens (Kopie)

Ref.-No.: 0105-6291-IKF

Processes and Methods (incl. Screening) : Life Sciences-HTS/HCS
Research Tools : Other
Nucleic Acid-, Protein and Cell-related Technologies : Protein related

A synthetic virus-like particle, comprising a lipid bilayer, that can be adjusted precisely to mimic biophysical features of various natural pathogens. The lipid bilayer can also be equipped with relevant molecules such as the SARS-CoV-2 spike protein ectodomain.

N-substituted pyridiniophosphines, processes for their preparation and their use

Ref.-Nr.: 0042-4938-LC-WA

Processes and Methods (incl. Screening) : Chemical
Research Tools : Other
New Materials

We offer a new family of cationic ligands, namely N-alkyl/aryl pyridiniophosphines. The ligands can be synthesized through a short, scalable, and highly modular route.

Evaluation of their electronic properties evidenced weak σ-donor and quite strong π-acceptor character when used as ancillary ligands.

Neuro-epithelial stem cells (NESC) capable of forming both central nervous system (CNS) and peripheral nervous system (PNS) neurons

Ref.-Nr.: 1012-4430-LI

Processes and Methods (incl. Screening)

File no.: MI-1012-4430-LI-ZE
(liked to MI-1012-5677-LI-ZE describing the generation of human neural microtissues from NECS cells)

A novel precursor population that forms electrophysiologically functional neurons more quickly than protocols starting with hPSCs.

New KDAC assay and selection system

Ref.-No.: 0803-5480-IKF

Analytics
Processes and Methods (incl. Screening) : Life Sciences-HTS/HCS
Medicine : Diagnostics
Nucleic Acid-, Protein and Cell-related Technologies : Protein related

We present an extremely sensitive, reliable and fast novel Lysine deacetylase (KDAC) assay, which can be performed in a continuous format. This novel KDAC assay is based on Firefly luciferase harboring an acetylation on an essential active site lysine, incorporated by genetic code expansion.

We furthermore offer a bacterial selection system that can help engineer KDACs. The selection system uses a reporter enzyme inactivated by lysine modifications at an essential active site residue, thereby ...

Parallel glycan synthesis on a membrane

Ref.-No.: 0401-5724-MG

Processes and Methods (incl. Screening) : Large Scale Production

A process for parallel synthesis of glycans on cellulose membranes by using a novel VaporSPOT method and an experimental setup that ensures controlled conditions suitable for glycosylation reactions.

Peptides and methods for carbon-carbon bond formation

Ref.-No.: 0706-5856-IKF

Processes and Methods (incl. Screening) : Chemical

One of biotechnology’s central goals is the synthesis of multicarbon compounds under mild and sustainable conditions from renewable resources. This requires biocatalysts and methods that enable selective C-C bond formation (carboligation) between two carbon units.

Moreover, optically active compounds can be used for optically resolving agents of medical or agriculture supplies such as 2-amino-1-butanol which is a starting material of the antituberculous drug ethambutol, diltiazem hydrochloride ...

Predicting concentrations, reactions rates and rate constants in biochemical networks

Ref.-No.: 0402-5469-MG

Analytics
IT / Software / Bioinformatics : Bioinformatics
Processes and Methods (incl. Screening) : Life Sciences-HTS/HCS
Nucleic Acid-, Protein and Cell-related Technologies : Protein related

A computer-implemented method that allows calculating concentration ranges of components within complex networks of biochemical reactions.

Stable, site-specific conjugation of His-tagged proteins via cobalt (III) carbonate reagents

Ref.-No.: 0105-5774-IKF

Analytics
Processes and Methods (incl. Screening) : Chemical
Processes and Methods (incl. Screening) : Life Sciences-HTS/HCS
Research Tools : Other
Nucleic Acid-, Protein and Cell-related Technologies : Protein related

This invention relates to means and methods for conjugating/attaching target molecules such as proteins to a label and/or carrier.

Substituted Imidazolium Sulfuranes and their use as novel Electrophilic Group-Transfer Reagents

Ref.-No.: 0042-5063-LC

Processes and Methods (incl. Screening) : Chemical
Research Tools : Other
New Materials

The ability of hypervalent iodine compounds to act as electrophilic group-transfer reagents has been extensively exploited during the last several years in a variety of synthetically useful transformations
like cyanations and alkynylations. Now it was found, that novel imidazolium sulfuranes, which are isolobal to I(III) species and also depict the key threecenter four-electron bond motive, can be used as an alternative platform for the development of new electrophilic group-transfer reagents.

Imidazolium ...

SUMOvera: A Truly Orthogonal Cleavage Module

Ref.-Nr.: 0402-5439-LI

Processes and Methods (incl. Screening) : Life Sciences-HTS/HCS
Research Tools : Other
Nucleic Acid-, Protein and Cell-related Technologies : Protein related

SUMOvera enables versatile purification strategies for broad applications in eukaryotic hosts.

Tet-inducible “gePSI” protein reversibly regulates eukaryotic ribosomal protein synthesis in a cell-type specific manner allowing to impose and release protein synthesis inhibition in a select subset of cells in mixtures

Ref.-No.: 0602-5504-LI

Processes and Methods (incl. Screening) : Life Sciences-HTS/HCS
Research Tools : Other
Nucleic Acid-, Protein and Cell-related Technologies : Nucleic acid related

Proteins are the main functional units within cells. Their regulated synthesis and degradation are crucial for controlling biological processes. In all cells, protein synthesis is used to respond to extra- and/or intracellular cues to remodel cellular function. Previous studies probing the role of protein synthesis have used chemical inhibitors, often common antibiotics, which are effective but lack functional and cell-type specificity when applied to a mixture of cells.

Unique multifunctional oligonucleotide enabling ultrafast RNA-protein interactions in cells and other uses thereof

Ref.-No.: 0214-5029-MG

Processes and Methods (incl. Screening) : Life Sciences-HTS/HCS
Research Tools : Other
Nucleic Acid-, Protein and Cell-related Technologies : Nucleic acid related
Nucleic Acid-, Protein and Cell-related Technologies : Protein related

Unique multifunctional oligonucleotide for the ultrafast transcriptome-wide identification of RNA-binding protein (RBP) targets.

Xenorhodopsin - a new inward-directed proton pump for an alternative optogentic approach

Ref.-No.: 0601-5295-MG

Processes and Methods (incl. Screening) : Life Sciences-HTS/HCS
Medicine : Therapeutics
Research Tools : Other
Nucleic Acid-, Protein and Cell-related Technologies : Cell related

The development of the cation channel channelrhodopsin-2 (ChR2) from Chlamydomonas reinhardtii by Prof. Ernst Bamberg and his colleagues, and its application to light-induced modulation of neurons paved the way for the field of optogenetics.
Over time the light-gated cation channel channelrhodopsin-2 (ChR2) has become an indispensable tool in neuroscience. Based on the pioneering work on ChR2 new variants have emerged, differing in their spectrum of light absorption, their kinetic ...